MCB2340C Section 04 General MicrobiologyModule 02 QuizAssignment ContentQuestion 1All these terms categorize microorganisms based on their temperature preference EXCEPT1.Obligate halophile2.Psychrophile3.Mesophile4.PsychrotrophQuestion 2If the heat-fixation step is omitted in a simple stain, what will happen?1.The dye will not be taken up by the cells2.The staining process will destroy the cells3.The cells can wash off the slide4.The cell morphology will changeQuestion 3Gram-negative cell walls contain a thin layer of peptidoglycan, whereas Gram-positive cell walls contain a thick layer of peptidoglycan.1. True2. FalseQuestion 4All the following organisms are expected to grow within an anaerobe jar EXCEPT1.Obligate anaerobe2.Aerotolerant anaerobe3.Obligate aerobe4.Facultative anaerobeQuestion 5If a bacterium were placed in a hypertonic solution prepared by the addition of sucrose, which of the following would happen to the bacterium?1.The bacterial cell would swell but not lyse because of the presence of the cell wall2.The bacterial cell would not change3.The bacterial cell would undergo osmotic lysis4.The bacterial cell would undergo plasmolysisQuestion 6All the following are environmental factors that affect microbial growth EXCEPT1.Temperature2.Osmotic pressure3.pH4.Cell wall typeQuestion 7A ___________ is used to remove the primary stain from the bacterial cell wall in a differential staining technique.1.Mordant2.Decolorizer3.Secondary stain4.CounterstainQuestion 8In the acid-fast stain, non-acid-fast bacteria appear blue under the microscope.1. True2. FalseQuestion 9An acidophile is an organism that has an optimal pH for growth below 5.5.1. True2.FalseQuestion 10All microorganisms use oxygen in their metabolism.1. True2. FalseQuestion 11The environmental conditions on human skin have NaCl levels slightly above that of most bacterial cells. What category of organism would most likely be part of the normal skin microflora?1.A halotolerant organism2.An obligate halophile3.An obligate anaerobe4.A psychrophileQuestion 12Capsule staining techniques stain the background and the vegetative cell of an incapsulated bacterium because the capsule doesn’t react with most stains.1. True2.FalseQuestion 13All these terms categorize microorganisms based on their temperature preference EXCEPT:1.Mesophile2.Psychrophile3.Psychrotroph4.ChemoautotrophQuestion 14All the following are found in most prokaryotic cells EXCEPT:1.cell membrane2.cytoplasm3.cell wall4.NucleusQuestion 15A ____________ is used in the Gram stain procedure to complex with the dye and trap it within the cell of a bacterium with a thick peptidoglycan cell wall?1.Mordant2.Decolorizer3.Counterstain4.Primary stainQuestion 16Gram positive cells appear purple after Gram staining whereas Gram negative cells appear pink.1. True2. FalseQuestion 17___________________ is used to stain the endospore in the endospore staining procedure.1.Methylene blue2.Crystal violet3.Malachite green4.SafraninQuestion 18Heat-fixation is performed in slide preparation in order to _________________________________.1.Fix the morphology of the organisms on the slide2.Encourage bacterial growth on the slide3.Inactivate and adhere microorganism placed on the slide4.Protect the bacteria by hardening them to prevent destruction by the staining procedureQuestion 19Heating or steaming a fixed smear with the primary stain, malachite green, enhances the penetration of the stain into this structure.1.Capsule2.Cell wall3.Chloroplast4.EndosporeQuestion 20A hypotonic solution is one with a solute concentration greater than the concentration which exists within the cell.1. True2. FalseQuestion 21If a bacterium were placed in a hypotonic solution prepared by the addition of sucrose, which of the following would happen to the bacterium?1.The bacterial cell would swell but not lyse because of the presence of a cell wall2.The bacterial cell would not change3.The bacterial cells will undergo osmotic lysis4.The bacterial cells will undergo plasmolysisQuestion 22The simple stain technique provides enough information about a microbe to identify its species reliably:1. True2. FalseQuestion 23As long as the normal enzymatic function is not compromised, a microorganism will grow across the range of pH.1. True2. FalseQuestion 24Organisms that require a lower level of molecular oxygen for their metabolism than what is typically found in the atmosphere, are called __________________?1.Microaerophiles2.Obligate aerobes3.Facultative anaerobes4.Aerotolerant anaerobesQuestion 25Where would you expect an obligate aerobe to grow in a fluid thioglycollate media tube?1.The upper part of the tube2.The lower part of the tube3.Throughout the tube4.The middle part of the tubeMCB2340C Module 02 QuizQuestion 1According to the binomial system of nomenclature, which of the following is the incorrect way to report a specific organism’s scientific name at any point in using it in an answer or a document.E. coliEscherichia coliEscherichia ColiAll the above answers are acceptableQuestion 2Isolated bacteria can be isolated from a mixed culture by all of the following techniques EXCEPT:Growing the sample in a nutrient broth enrichment cultureStreak platingSerial dilution followed by spread platingSerial dilution followed by pour platingQuestion 3The following method can be used to isolate a specific bacterial type from a mixed culture?Gram stainingEnrichment cultureAcid-fast stainingSerial dilution followed by spread platingQuestion 4The group of organisms that includes Saccharomyces cerevisiae (a yeast) and Penicillium aspergillus ( amold) is known as _______AlgaeBacteriaFungiVirusesQuestion 5Which is found in animal cells, but not plant or algae cells:Cell membraneChloroplastsCentral vacuoleCentriolesQuestion 6Which is found in plant or algae cells but not in animal cells:Cell membraneMitochondriaCell wallPeroxisomesQuestion 7Helminths are included in the study of microbiology becauseThey are all mammalian parasitesThey are mostly microscopic, and that is the only way they can be studiedThe eggs and larvae are mostly microscopic, and that is the easiest way to identify themThey are often found in a mixed infection which includes various species of microbesQuestion 8Which is a symbiotic relationship that benefits both members of the relationship?MutualismParasitismCommensalismAmensalismQuestion 9NaegleriafowleriGiardia lambliaEuglena gracilisBlastomycesdermatitidisQuestion 10Isolated bacteria can be isolated from a mixed culture by all of the following techniques EXCEPT:Streak platingLoop dilution pour platingGram stainingSerial dilution followed by spread platingQuestion 11The binomial system of nomenclature states that the correct way to report a specific organism’s scientific name the first time you use it in an answer or a document is:Escherichia coliE. coliEscherichia c.escherichia coliQuestion 12All animals, most bacteria, fungi, and protozoans that use organic compounds as the source of both carbon and energy are referred to as ___________________.PhotoautotrophsChemoautotrophsPhotoheterotrophsChemoheterotrophsQuestion 13An example of a bacterial virus isEscherichia coliBacteriophage T4InfluenzavirusAdenovirusQuestion 14Bacterial replication is accomplished primarily byMitosisMeiosisCytokinesisBinary fissionQuestion 15Organized communities of many different types of microbes growing on surfaces are called ____________.ColoniesBiofilmsBiospheresFloraQuestion 16These eukaryotes can have a complicated lifestyle including a fertilized egg, larvae, and adult stage and can involve several intermediate hosts before the definitive host for the adult parasite:FungiProtozoansHelminthsAll the above answersQuestion 17Which of the following is most likely to be the most frequently used portals of entry for a pathogen?Mucosal membranes of the gastrointestinal and respiratory tractsBroken skinGenitourinary tractConjunctiva of eyesB and D.Question 18The so-called “red tide” is generally caused by which of the following organisms?LichensBacteriaDiatomsAlgaeQuestion 19According to the binomial system of nomenclature, escherichia coli is the correct way to report this organism’s name the first time you use it in an answer or a document. After that point, you can abbreviate it to e-coli.TrueFalseQuestion 20The correct sequence of stages in the multiplication of animal viruses isAttachment, penetration, uncoating, biosynthesis, assembly, releasePenetration, attachment, assembly, uncoating, biosynthesis, releaseAttachment, uncoating, penetration, biosynthesis, assembly, releasePenetration, uncoating, adsorption, biosynthesis, assembly, releaseQuestion 21Bacterial stains are commonly used for all the following EXCEPT:The treatment of diseaseThe diagnosis of diseaseThe pathogen identification processThe classification of organismsQuestion 22Binary fission is the process used by which of these organisms for cell division?BacteriaHelminthsFungiAlgaeQuestion 23The subgroup of fungi that are used as food (edible mushrooms, morels, and truffles) are calledBasidiomycotaAscomycotaLichensZygomycotaQuestion 24A bacteriophage is an example of a complex bacterial virus which is neither purely helical or completely icosahedral and has protein tails and must always contain both DNA and RNA.TrueFalseQuestion 25Bacterial stains are commonly used for all the following EXCEPT:The pathogen identification processThe diagnosis of diseaseThe classification of organismsThe treatment of disease
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